What is Isoleucine 73-32-5?
L-Isoleucine (CAS 73-32-5) is one of the three essential branched-chain amino acids (BCAAs), along with leucine and valine. It plays a critical role in muscle metabolism, energy regulation, and protein synthesis, making it a key ingredient in sports nutrition, dietary supplements, clinical nutrition, and animal feed formulations.
For B2B buyers, including nutraceutical brands, sports nutrition manufacturers, pharmaceutical companies, and feed producers, bulk isoleucine provides a high-value, functional amino acid raw material suitable for large-scale production and formulation flexibility.
Main Active Ingredients & Technical Profile
|
Component |
Specification |
Function |
|
L-Isoleucine |
≥98.5% / ≥99% |
Active amino acid |
|
Moisture |
≤1.0% |
Stability control |
|
Heavy Metals |
≤10 ppm |
Safety compliance |
|
Residue on Ignition |
≤0.1% |
Purity indicator |
|
Optical Rotation |
Conforms |
Identity verification |
|
pH (1% solution) |
5.5–7.0 |
Formulation compatibility |
COA
|
Analysis |
Specification |
Result |
|
Physical Description |
|
|
|
Appearance |
White crystals or crystalline powder |
Complies |
|
PH |
5.5~7.0 |
6.06 |
|
Specific rotation[a]D25 |
+38.9°~+41.8° |
40.1° |
|
Organic volatile |
Complies |
Complies |
|
Chemical Tests |
|
|
|
Idenlification(IR) |
Concordant with the reference spectrum |
Complies |
|
Assay |
98.5%~101.5% |
100.3% |
|
Residual on ignition |
0.3% Max |
0.15% |
|
Loss on drying |
0.3% Max |
0.12% |
|
Chloride(Cl) |
0.05% Max |
Complies |
|
Sulfate(SO4) |
0.03% Max |
Complies |
|
Iron(Fe) |
30.0ppm Max |
Complies |
|
Arsenic(As) |
1.5ppm Max |
Complies |
|
Heavy metal (Pb) |
15.0ppm Max |
Complies |
|
Conclusion |
Complies with the USP24 Standard. |
|
|
General Status |
Non-GMO, Non-irradiation, ISO. |
|
|
Packing and Storage |
||
|
Packing: Pack in paper-carton and two plastic-bags inside. |
||
|
Shelf life: 2 year when properly stored. |
||
|
Storage: Store in a well-closed place with constant low temperature and no direct sun light. |
||
Key Benefits of L-Isoleucine

✔ Supports Muscle Energy Metabolism
Helps regulate energy supply during physical activity
✔ Promotes Muscle Recovery
Works synergistically with leucine and valine in muscle repair processes
✔ Maintains Nitrogen Balance
Supports protein metabolism and muscle preservation
✔ Enhances Endurance Performance
Commonly used in sports nutrition formulations
✔ Suitable for Clinical Nutrition
Used in amino acid infusion and recovery formulas
Core Applications
|
Application Field |
Practical Example |
Main Function |
Application Description |
|
Sports Nutrition |
BCAA powders, recovery drinks |
Muscle recovery |
Core ingredient in muscle recovery and endurance formulas. |
|
Dietary Supplements |
Capsules, tablets |
Amino acid support |
Used in daily essential amino acid supplementation. |
|
Clinical Nutrition |
Infusion solutions |
Medical recovery |
Supports amino acid balance in clinical settings. |
|
Functional Foods |
Protein bars, drinks |
Nutritional enhancement |
Improves amino acid profile of functional foods. |
|
Animal Feed |
Livestock feed additives |
Growth support |
Enhances protein utilization and feed efficiency. |

Production & Supply Capability
|
Packaging Type |
Description |
|
25 kg drum |
Standard export packaging |
|
20–25 kg bag |
Industrial bulk supply |
|
1–5 kg sample bag |
R&D use |
|
Custom packaging |
OEM/ODM available |
Supplied by Purea Biological Technology Co., Ltd. with:
✔ Stable bulk production
✔ COA / MSDS / HPLC documentation
✔ Global logistics support
Market Trends & Demand Outlook
The global demand for L-Isoleucine is increasing due to:
·Growth of sports nutrition industry
·Rising demand for BCAA-based supplements
·Expansion of clinical amino acid therapy
·Increasing focus on high-protein diets
�� BCAAs are among the fastest-growing amino acid segments globally
How to Choose High-Quality L-Isoleucine
B2B buyers should evaluate:
1.Purity ≥99% for premium applications
2.Fermentation origin (preferred)
3.Low heavy metal content
4.Stable particle size distribution
5.Reliable supplier documentation
Choosing Purea Biological Technology Co., Ltd. ensures:
✔ Consistent quality
✔ Stable supply chain
✔ Technical support for formulation
FAQ
Q1: What is L-Isoleucine used for?
A: It is widely used in sports nutrition, supplements, clinical nutrition, and feed applications.
Q2: Is L-Isoleucine an essential amino acid?
A: Yes, it is an essential amino acid and part of the BCAA group.
Q3: Can it be used in protein powders?
A: Yes, it is commonly used in BCAA and protein supplement formulations.
Q4: What is the recommended purity for food applications?
A: Typically ≥98.5% or ≥99% purity.
Q5: How should it be stored?
A: Store in a cool, dry, sealed environment away from moisture.
Where to buy Isoleucine 73-32-5?
Pure Biological provides high-quality Isoleucine 73-32-5, supports customer OEM customization, from samples to bulk goods, can respond quickly, and has a good service foundation.
Please contact us by email for more information.Email:sales@pureabio.com
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